Journal: Food Science & Nutrition

Article Title: Vitamin D ameliorates asthma‐induced lung injury by regulating HIF‐1α/Notch1 signaling during autophagy

doi: 10.1002/fsn3.2880

Figure Lengend Snippet: Comparison of airway hyperresponsiveness (AHR) in two groups of mice

Article Snippet: The following reagents were used in the present study: OVA (batch No.: 9006‐59‐1; Solarbio Life Science); acetylcholine chloride (batch No.: 2260‐50‐6; Selleck); hematoxylin–eosin (HE) staining solution (batch No.: P032IH; Auragene Bioscience Co.); Masson and periodic acid–Schiff (PAS) staining solutions (batch Nos.

Techniques: Comparison

Journal: Biochimica et biophysica acta. Molecular cell research

Article Title: When "No-Smoking" is not enough: Hypoxia and nicotine acetylcholine receptor signaling may drive lung adenocarcinoma progression in never-smokers.

doi: 10.1016/j.bbamcr.2022.119302

Figure Lengend Snippet: Fig. 4. Role of nAChR-α7 mediated pathway in regulating the expression of HIFs and migration in A549 and NCI-H460 cancer cells in hypoxia: (A) Acetylcholine detection assay. (i) Acetylcholine standard curve (ii) Time point analysis of Acetylcholine concentration in normoxia and 0.2% hypoxia (B) Protein expression of (i) HIF-1α was increased with Acetylcholine and HIF-2α remained unchanged in presence of Acetylcholine chloride (ACh) (UT = untreated) in A549 (left panel) and NCI- H460 (right panel) (iii) HIF-1α expression increased while HIF-2α protein level was unaffected in presence of acetylcholine nAChR-α7 agonist nicotine in A549 cells . (iii) Repression of HIF-1α and pAKT, but not HIF-2α and total AKT protein levels was observed in presence of α-BTX . Both β–actin and total protein (not shown here) were used as loading controls. (C)(i) HIF-1α protein is reduced in the presence of AKT inhibitor MK-2206 compared to untreated controls (ii) A reduction, although insignificant, in the CHRNA7 transcripts was observed in the presence of AKT inhibitor. (D) Wound healing assay. (i) Photomicrograph of wounded A549 cells treated with 10 nM and 25 nM α-BTX in hypoxic condition at different timepoints. (ii) Effect of α-BTX plotted as percentage of wound closure against time (0 h). (E) Migration assay. (i) Photomicrograph of migrated A549 cells 16 h after treatment with 25 nM α-BTX in hypoxic condition (ii) Plot representing percentage of A549 cell migrated in untreated and α-BTX treated conditions. The error bars indicate standard deviations. * represents p < 0.05 relative to its untreated (UT) counterpart. A black line has been added to show the break in the western blot.

Article Snippet: Various treatment conditions such as Nicotine (Sigma, USA), α-Bungarotoxin (αBTX) (Sigma, USA), AKT inhibitor MK-2206 (Caymen, USA) and Acetylcholine chloride (Ach) (Invitrogen, USA) in doses as indicated in the text were given in normoxia and in 0.2% hypoxia.

Techniques: Expressing, Migration, Detection Assay, Concentration Assay, Wound Healing Assay, Western Blot

Journal:

Article Title: Effects of endothelial impairment by saponin on the responses to vasodilators and nitrergic nerve stimulation in isolated canine corpus cavernosum

doi: 10.1038/sj.bjp.0702623

Figure Lengend Snippet: Modifications by L-NOARG (10 μM, 5 in the parenthesis) and L-NOARG plus L-arginine (L-Arg., 1 mM) (left figure) and by indomethacin (1 μM) (right) of the relaxant response to acetylcholine in corpus cavernosum strips contracted with phenylephrine. The ordinate represents relaxations relative to those induced by 0.1 mM papaverine. Significantly different from the value with L-NOARG, aP<0.01; bP<0.05 (Tukey's test). ‘n' denotes the number of strips from separate dogs. Vertical bars represent s.e.mean.

Article Snippet: Drugs used were N G -nitro- L -arginine ( L -NOARG), vasoactive intestinal peptide (VIP), substance P, bradykinin (Peptide Institute, Minoh, Japan), L -arginine (Nacalai Tesque, Kyoto, Japan), acetylcholine chloride (Daiichi Co., Osaka, Japan), sodium nitroprusside (Merck, Darmstadt, Germany), ATP, aminophylline, indomethacin, physostigmine sulphate, 1-phenylephrine hydrochloride (Sigma Chemical, St. Louis, MO, U.S.A.), atropine sulphate, thiopental (Tanabe, Osaka), bretylium tosylate (Glaxo Wellcome, NC, U.S.A.), tetrodotoxin (Sankyo Co., Tokyo), Ca 2+ ionophore A23187 (C. H. Boehringer Ingelheim, Elmsford, NY, U.S.A.) and papaverine hydrochloride (Dainippon Co., Osaka).

Techniques:

Journal:

Article Title: Effects of endothelial impairment by saponin on the responses to vasodilators and nitrergic nerve stimulation in isolated canine corpus cavernosum

doi: 10.1038/sj.bjp.0702623

Figure Lengend Snippet: Modifications by L-NOARG (100 μM, 4 in the parenthesis), L-NOARG plus ODQ (1 μM) (+ODQ) and L-NOARG plus K+ (L-NOARG/K+) of the relaxant response to acetylcholine in corpus cavernosum strips. The strips were contracted with phenylephrine except for L-NOARG/K+ in which the preparations were contracted with K+ (15–20 mM), the concentration being adjusted to match the contraction induced by phenylephrine under control conditions. The ordinate represents relaxations relative to those elicited by 0.1 mM papaverine. Significantly different from control, aP<0.01, bP<0.05; significantly different from the value with ODQ, cP<0.01; significantly different from the value with 100 μM L-NOARG dP<0.01 (Tukey's test). ‘n' denotes the number of strips from separate dogs. Vertical bars represent s.e.mean.

Article Snippet: Drugs used were N G -nitro- L -arginine ( L -NOARG), vasoactive intestinal peptide (VIP), substance P, bradykinin (Peptide Institute, Minoh, Japan), L -arginine (Nacalai Tesque, Kyoto, Japan), acetylcholine chloride (Daiichi Co., Osaka, Japan), sodium nitroprusside (Merck, Darmstadt, Germany), ATP, aminophylline, indomethacin, physostigmine sulphate, 1-phenylephrine hydrochloride (Sigma Chemical, St. Louis, MO, U.S.A.), atropine sulphate, thiopental (Tanabe, Osaka), bretylium tosylate (Glaxo Wellcome, NC, U.S.A.), tetrodotoxin (Sankyo Co., Tokyo), Ca 2+ ionophore A23187 (C. H. Boehringer Ingelheim, Elmsford, NY, U.S.A.) and papaverine hydrochloride (Dainippon Co., Osaka).

Techniques: Concentration Assay, Control

Journal:

Article Title: Effects of endothelial impairment by saponin on the responses to vasodilators and nitrergic nerve stimulation in isolated canine corpus cavernosum

doi: 10.1038/sj.bjp.0702623

Figure Lengend Snippet: Tracings of the response to acetylcholine (ACh, 10 nM–10 μM) as affected by L-NOARG (100 μM), L-NOARG plus ODQ (1 μM) and L-NOARG plus ODQ (K+) in a corpus cavernosum strip. The preparation was contracted with phenylephrine in the upper three tracings but with K+ (15 mM) in the bottom tracing. PA represents 0.1 mM papaverine that produced the maximal relaxation. Numbers indicate the concentrations of the drugs in −log[M].

Article Snippet: Drugs used were N G -nitro- L -arginine ( L -NOARG), vasoactive intestinal peptide (VIP), substance P, bradykinin (Peptide Institute, Minoh, Japan), L -arginine (Nacalai Tesque, Kyoto, Japan), acetylcholine chloride (Daiichi Co., Osaka, Japan), sodium nitroprusside (Merck, Darmstadt, Germany), ATP, aminophylline, indomethacin, physostigmine sulphate, 1-phenylephrine hydrochloride (Sigma Chemical, St. Louis, MO, U.S.A.), atropine sulphate, thiopental (Tanabe, Osaka), bretylium tosylate (Glaxo Wellcome, NC, U.S.A.), tetrodotoxin (Sankyo Co., Tokyo), Ca 2+ ionophore A23187 (C. H. Boehringer Ingelheim, Elmsford, NY, U.S.A.) and papaverine hydrochloride (Dainippon Co., Osaka).

Techniques: Stripping Membranes, Produced

Journal:

Article Title: Effects of endothelial impairment by saponin on the responses to vasodilators and nitrergic nerve stimulation in isolated canine corpus cavernosum

doi: 10.1038/sj.bjp.0702623

Figure Lengend Snippet: Concentration-response curves of acetylcholine (left figure) and sodium nitroprusside (SNP) (right) in corpus cavernosum strips before (control) and after treatment with saponin. The strips were contracted with phenylephrine. The ordinate represents relaxations relative to those induced by 0.1 mM papaverine. Significantly different from control, aP<0.001 (unpaired t-test). ‘n' denotes the number of strips from separate dogs. Vertical bars represent s.e.mean.

Article Snippet: Drugs used were N G -nitro- L -arginine ( L -NOARG), vasoactive intestinal peptide (VIP), substance P, bradykinin (Peptide Institute, Minoh, Japan), L -arginine (Nacalai Tesque, Kyoto, Japan), acetylcholine chloride (Daiichi Co., Osaka, Japan), sodium nitroprusside (Merck, Darmstadt, Germany), ATP, aminophylline, indomethacin, physostigmine sulphate, 1-phenylephrine hydrochloride (Sigma Chemical, St. Louis, MO, U.S.A.), atropine sulphate, thiopental (Tanabe, Osaka), bretylium tosylate (Glaxo Wellcome, NC, U.S.A.), tetrodotoxin (Sankyo Co., Tokyo), Ca 2+ ionophore A23187 (C. H. Boehringer Ingelheim, Elmsford, NY, U.S.A.) and papaverine hydrochloride (Dainippon Co., Osaka).

Techniques: Concentration Assay, Control

Journal:

Article Title: Carvedilol improves endothelium-dependent vasodilation in patients with dilated cardiomyopathy

doi: 10.1136/hrt.2006.087999

Figure Lengend Snippet: Figure 1 Comparison of the forearm blood flow response to acetylcholine (left) or sodium nitroprusside (right) administration in the carvedilol group (top) and the control group (bottom). Comparison of the forearm blood flow response to acetylcholine administration after the infusion of l‐NMMA in the carvedilol group (left top) and the control group (left bottom).

Article Snippet: 4 The effects of infusion of acetylcholine (ACh; Daiichi Pharmaceutical, Tokyo, Japan) and sodium nitroprusside (SNP; Maruishi Pharmaceutical, Osaka, Japan) on FBF were also measured.

Techniques: Comparison, Control